Welcome to pep2pro - from peptide to proteome



pep2pro is a further development of AtProteome [link] and provides proteome information on Arabidopsis thaliana. The underlying proteome analysis database allowed integrating and analysing the large proteome datasets listed below (described in Baerenfaller et al., 2011 [Link]) and the website shows all the information about the protein identifications with a proteogenomic mapping of the peptides onto the genome. The pep2pro TAIR9 wos dataset is provided as the default dataset. The other datasets can be selected from the drop-down menu 'Choose the Assembly' and depending on the information available, the corresponding submenus will appear. The datasets marked with an asterisk are included in the MASCP Gator (http://gator.masc-proteomics.org).

For finding a protein in a dataset, click on 'Protein Search' and enter the search arguments. These can either be an ATG number (first 9 characters without the splice information, e.g. AT2G39730), or a word from the annotation line (e.g. activase). One can also enter several ATG numbers or several search terms in parallel, separated with space, comma, or semicolon). In order to retrieve more information about the identification, click on the protein of interest.

Please see the [help file] to get more information and to get an introduction to the other features of the website.

Dataset Spectra specFDR Distinct
peptides
Loci
TAIR10 database 27'416
pep2pro TAIR10 wos (without single hits) 2'669'654 0.7% 141'934 14'582
TAIR9 database 27'379
Plastid Proteome Assembly in absence of Toc159 *)
Sylvain Bischof, Katja Baerenfaller, ThomasWildhaber, Raphael Troesch, Pierre-Alexandre Vidi, Bernd Roschitzki, Matthias Hirsch-Hoffmann, Lars Hennig, Felix Kessler, Wilhelm Gruissem and Sacha Baginsky
Plastid Proteome Assembly without Toc159: Photosynthetic Protein Import and Accumulation of N-Acetylated Plastid Precursor Proteins.
The Plant Cell November 2011 [Link]
296'571 0.52% 29'524 5'159
Comparative phosphoproteome study of the STN8 kinase mutant *)
Reiland S., Finazzi G., Endler A., Willig A., Baerenfaller K., Grossmann J., Gerrits B., Rutishauser D., Gruissem W., Rochaix J-D. and Baginsky S.
Comparative phosphoproteome profiling reveals a function of theSTN8 kinase in fine-tuning of cyclic electron flow (CEF).
PNAS 2011 [Link]
1applies to phosphoproteome data
15'492 1 0.15% 1 3'589 1 1'738 1
AtProteome TAIR9 803'530 1.0% 87'630 13'290
AtProteome TAIR9 extended 1'066'363 1.0% 97'010 14'378
pep2pro TAIR9 2'664'441 0.9% 144'824 18'111
pep2pro TAIR9 wos *) (without single hits)
Katja Baerenfaller, Matthias Hirsch-Hoffmann, Julia Svozil, Roger Hull, Doris Russenberger, Sylvain Bischof, Qingtao Lu, Wilhelm Gruissem and Sacha Baginsky
pep2pro: a new tool for comprehensive proteome data analysis to reveal information about organ-specific proteomes in Arabidopsis thaliana
Integr. Biol., 2011, 3, 225-237 [Link]
2'660'852 0.7% 141'235 14'522
TAIR8 database 27'235
Arabidopsis thaliana leaf wound proteome *)
Gfeller A., Baerenfaller K., Loscos J., Chételat A., Baginsky S. and Farmer E.
Jasmonate controls polypeptide patterning in undamaged tissue in wounded Arabidopsis leaves.
Plant Physiology 2011 [Link]
216'896 1.0% 29'908 6'530
AtProteome TAIR8 799'292 1.0% 86'763 13'104
AtProteome TAIR8 extended 1'062'435 1.0% 96'145 14'195
Large-scale phosphoproteome analysis of Arabidopsis thaliana shoots
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., and Baginsky S.
Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks.
Plant Physiology 2009 [Link]
4'905
TAIR7 database 27'029
AtProteome TAIR7 (original)
Baerenfaller K., Grossmann J., Grobei M.A., Hull R., Hirsch-Hoffmann M., Yalovsky S., Zimmermann P., Grossniklaus U., Gruissem W. and Baginsky S.
Genome-scale proteomics reveals Arabidopsis thaliana gene models and proteome dynamics.
Science 2008 [Link]
790'181 0.9% 86'456 13'029
AtProteome TAIR7 803'577 1.0% 86'966 13'154
Pollen proteome map of Arabidopsis thaliana TAIR7
Grobei MA et al.
Deterministic protein inference for shotgun proteomics data provides new insights into Arabidopsis pollen development and function
Genome Research, 2009
4'436
*) The datasets marked with an asterisk are included in the MASCP Gator (http://gator.masc-proteomics.org).

News

31 March 2012, TPP Search: New search functionality made available displaying all theoretical tryptic (TTP) peptides for a protein with minimum length of 6 amino acids and between 400 and 6000 Da and with a flag indicating whether they are unambiguous

01 February 2012, Spectrum Summary: introduction of the spectrum count normalized to the total number of spectra in each sample

29 November 2011, Public release of the 'Plastid Proteome Assembly in the Absence of Toc159' dataset upon publication in Plant Cell

01 July 2011, Public release of the 'Comparative phosphoproteome study of the STN8 kinase mutant' dataset upon acceptance of the manuscript by PNAS

21 June 2011, Public release of the 'Arabidopsis thaliana leaf wound proteome' dataset upon acceptance of the manuscript by Plant Physiology

10 March 2011, pep2pro links to Genevestigator in the protein search section

04 March 2011, Release of the 'pep2pro TAIR10 wos' dataset

January 2011, Public release of the pep2pro website and database